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KMID : 1140120100150010083
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Cancer Prevention Research
2010 Volume.15 No. 1 p.83 ~ p.91
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Induction of Apoptosis and Inhibition of Prostaglandin E2 Production by Proteoglycan of Phellinus linteus in U937 Human Leukemia Cells
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Park Cheol
Hyun Sook-Kyung
Hwang Hye-Jin
Choi Sung-Hyun
Choi Young-Hyun
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Abstract
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Various mushroom polysaccharides and polysaccharides-protein complexes are known to possess anti-tumor and immunomodulating effects. Here, we demonstrated that proteoglycan etracted from Phellinus linteus has a potent anti-tumor and/or anti-inflammatory activity. Proteoglycan treatment resulted in a concentration-dependent growth inhibition by including apoptosis, which could be proved by DAPI staining, agarose gel electrophoresis and flow cytometry analysis. The increase in apoptosis induced by proteoglycan was correlated with up-regulation of pro-apoptotic Bax and Bad expression, and downregulation of anti-apoptotic Bcl-2 and Bcl-xL expression. Proteoglycan inhibited the levels of IAP family members such as XIAP and cIAP-1 and induced the proteolytic activation of caspase-3, and a concomitant degradation of β-catenin. In addition, it was found that proteoglycan treatment markedly decreased the levels of cyclooxygenases (COX)-2 mRNA and protein expression without significant changes in the expression of COX-1, which was correlated with a decrease in prostaglandin E2 synthesis. Taken together, these findings provide important new insights into the possible molecular mechanisms of the anti-cancer activity of proteoglycan.
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KEYWORD
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Phellinus linteus, Proteoglycan, Apoptosis, Prostaglandin E2
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